Description
Autologous Minced Cartilage Implantation is a treatment method for small cartilage lesions, in which cartilage from a non weight bearing area or from the defect margins is harvested, cut into ~1mm3 pieces, mixed with plasma products, and implanted into the defect. Minced Cartilage Implantation has already been successfully applied in small cartilage defects in otherwise healthy persons. The ideas underlying this method are, on the one hand, to activate the chondrocytes within the implanted cartilage pieces. On the other hand, mincing increases the cartilage surface, which should facilitate the migration of chondrocytes into the surrounding tissue. In a pilot study, we aim to evaluate different cartilage mincing methods in vitro regarding their effects on parameters that are relevant for cartilage regeneration. These include, for example, viability and migration of chondrocytes, expression of chondrocyte differentiation markers, cytokine production, and mechanical properties of cartilage. We also aim to optimize this method, for example, by combination with therapeutic substances, such as glucocorticoids or hyaluronic acid. In addition, we want to analyze whether Autologous Minced Cartilage Implantation could be a potential therapeutic option for the treatment of degenerative cartilage disorders, such as osteoarthritis. For our studies, we are planning to use human cartilage from informed consenting patients undergoing knee replacement surgeries. The tissue is routinely removed during these surgical procedures and would otherwise be discarded as „surgical waste“. For mechanical studies, we are planning to use osteochondral biopsies from bovine knees that will be obtained from a local abbatoir.
Details
| Duration | 01/08/2021 - 31/12/2025 |
|---|---|
| Funding | Unternehmen |
| Department | |
| Principle investigator for the project (University for Continuing Education Krems) | Univ.-Prof. Dr. Stefan Nehrer, MSc |
Publications
Moser, L.B.; Fickert, S.; Pitzek, S.; Bauer, C.; Neubauer, M.; Dammerer, D.; Zantop, T.; Landgraeber, S.; Nehrer, S. (2025). Minced cartilage for focal cartilage defects-A comprehensive systematic review of surgical techniques in clinical studies, animal studies and basic research studies. Knee Surgery, Sports Traumatology, Arthroscopy (KSSTA), 3: 1-21
Moser, L.B.; Bauer, C.; Otahal, A.; Kern, D.; Dammerer, D.; Zantop, T.; Nehrer, S. (2023). Mincing bovine articular cartilage with commercially available shavers reduces the via-bility of chondrocytes compared to scalpel mincing. J Exp Orthop., 10(1): doi: 10.1186/s40634-023-00661-5.
Lectures
Integration von additiver Fertigung und in-silico Simulation: Design und Finite-Element-Analyse von 3D-druckbaren Meniskusimplantaten
Digital Orthopedics Award by OPED, 12/12/2024
3D bioprinted silk fibroin-based hydrogels for meniscus tissue engineering
6th MuSkITYR symposium, 04/11/2024
Advancements in the Development of 3D-Printed Meniscal Implants: Virtual Prototyping and Biomechanical Assessment
7th ICRS Summit, 27/09/2024
Meniskus Bioprinting: Biomaterialeigenschaften & Finite Elemente Analyse
AGA Denkfabrik, 06/07/2024
Biomechanische in-silico Evaluierung eines 3D gedruckten Meniskus-Implantates mittels Finiter Element Analyse
GOTS, 39. Jahreskongress, 20/06/2024
3D printed shock absorbers for the human knee joint
Pint of Science, 14/05/2024
Comparison of two silk fibroin-based bioinks for meniscus bioprinting
OARSI World Congress on Osteoarthritis, 18/04/2024
Enhancing meniscus implant biomechanics: an in-silico analysis through 3D printing
OARSI World Congress on Osteoarthritis, 18/04/2024
Formulation and biomechanical characterisation of a silk fibroin-based bioink for meniscus replacement via bioprinting
LBG Meeting for Health Sciences 2023, 02/11/2023
3D-Meniskus-Regeneration: Wie aus µCT Scans ein 3D gedruckter Meniskus werden kann
ICCB 2023, 20/09/2023
Formulation and biomechanical characterisation of a silk fibroin-based bioink for meniscus replacement via bioprinting
17th World Congress ICRS 2023, 10/09/2023
3D-Meniscus-Regeneration: From µCT imaging to 3D printing
17th World Congress ICRS 2023, 09/09/2023
3D-Meniskus-Regeneration: Wie aus µCT Scans ein 3D gedruckter Meniskus werden kann
GOTS 38th annual congress, 15/06/2023
Finite Element Analysis
AGA Denkfabrik, 26/05/2023
Menisc-3D-SilkPrint
M3d+it Conference, 02/12/2022
The potential of mulberry and non-mulberry silk fibroin bioinks for meniscus regeneration by 3D-bioprinting’
Joint KMM-VIN / ViCEM / ESB cross-disciplinary workshop, 22/09/2022
Cartilage derived extracellular matrix incorporated silk fibroin hybrid scaffolds for endochondral ossification mediated bone tissue regeneration
TERMIS EU 2022, 28/06/2022
The potential of mulberry and non-mulberry silk fibroin blends as bioinks for meniscus regeneration by 3D-bioprinting
TERMIS EU 2022, 28/06/2022
The potential of mulberry and non-mulberry silk fibroin blends as bioinks for meniscus regeneration by 3D-bioprinting
Summer school ‘Frontiers in Regenerative Medicine’, 23/05/2022
