Advanced Flow Cytometric Characterization of Extracellular Vesicles

In this project, we will develop advanced methods for the flow cytometric characterization of extracellular vesicles. We will (i) establish novel approaches for the simultaneous detection of surface markers on extracellular vesicles using the MACSPlex technology; (ii) develop flow cytometric methods for the characterization of vesicles in the size range below 200 nm (binding to magnetic beads, fluorescence triggering), (iii) set up a method for the flow cytometric characterization of neutrophil extracellular traps, and (iv) characterize the role of extracellular vesicles in immunomodulation.