Characterisation of Blood Cell-derived Exosomes with Gas-Phase Electrophoretic Mobility Molecular Analysis

Aim of the project is to set up standardized protocols for the isolation of highly pure extracellular vesicle (EV) fractions from complex biological matrices, such as human blood or plasma, and to investigate the application of Nano Electrospray Gas-phase Electrophoretic Mobility Molecular Analysis (nES-GEMMA) for the characterization of EVs, in particular exosomes, with respect to quantification, which is potentially superior to indirect quantification based on protein concentration as used to date. We will assess for the first time whether protein and lipid content are qualitatively influenced by the size of extracellular vesicles by collecting size separated vesicles via an electrostatic nanometer aerosol sampler. Furthermore, eventual modifications in the composition of vesicles obtained from physiological and different pathological settings will be monitored.

Funding: Coutry of Lower Austria

Collaboration:

University of Technology Vienna

Univ.-Prof. Dr. Günter Allmaier

Dr. Victor Weiss