Description
The chronic degenerative disease osteoarthritis affects around 60% of people worldwide and is characterized by a progressive degradation of the cartilage. Patients suffer from pain, invalidity and dramatic loss of life quality. Besides medicamentous treatments, transplantation of autologous chondrocytes, the only cells within the cartilage, or total knee replacement by an implant are the only therapies to improve the clinical picture of osteoarthritis. A new regenerative approach is the use of patient´s blood derivatives. Platelet-rich plasma (PRP) and hypACTTM serum are the two most prominent among these. These products are an enrichment of various factors (especially growth factors) which accelerate the healing process of cartilage defects. OrthoSera GmbH has developed the CE marked device hypACTTM inject. With this syringe blood from the patient can be retrieved, centrifuged and processed within the same medical procedure. The obtained hypACTTM serum within hypACTTM inject can then directly be injected into the defective side. Together with exosomes and apoptotic bodies, microvesicles form the group of the so called extracellular vesicles which represent a novel mechanism by which pathogenic signals are communicated among different cell types. Extracellular vesicles transport bioactive molecules from one cell to an other leading to a biological response within the target cell. The aim of this project is to isolate and characterize microvesicles from the blood derivatives PRP and hypACTTM serum in order to determine their regenerative potential to fight osteoarthritis.
Cooperation partner:
Details
| Duration | 01/01/2018 - 29/02/2020 |
|---|---|
| Funding | Bundesländer (inkl. deren Stiftungen und Einrichtungen) |
| Program |
Technologieförderung NÖ
|
| Department | |
| Principle investigator for the project (University for Continuing Education Krems) | Assoz. Prof. Mag. Andrea De Luna, PhD |
| Project members |
Team
Publications
Otahal, A.; Kramer, K.; Kuten-Pella, O.; Moser, L.; Neubauer, M.; Lacza,Z.; Nehrer, S.; De Luna, A. (2021). Effects of Extracellular Vesicles from Blood-Derived Products on Osteoarthritic Chondrocytes within an Inflammation Model. Int J Mol Sci., 22(13): 7224
Otahal, A.; Kuten-Pella, O.; Kramer, K.; Neubauer, M.; Lacza, Z.; Nehrer, S.; De Luna, A. (2021). Functional repertoire of EV-associated miRNA profiles after lipoprotein depletion via ultracentrifugation and size exclusion chromatography from autologous blood products. Sci Rep., 12;11(1): 5823
Otahal, A.; Kramer, K.; Kuten-Pella, O.; Weiss, R.; Stotter, C.; Lacza, Z.; Weber, V.; Nehrer, S.; De Luna, A. (2020). Characterization and chondroprotective effects of extracellular vesicles from plasma- and serum-based autologous blood-derived products for osteoarthritis therapy. Front Bioeng Biotechnol, Sep 25: 584050
Lectures
miRNAs enriched in extracellular vesicles in plasma and serum based autologous blood derived products for osteoarthritis therapy
YIRM, Krems, Österreich, 13/12/2019
miRNAs enriched in extracellular vesicles in plasma and serum based autologous blood derived products for osteoarthritis therapy
ASEV-GSEV joint meeting, Freising, Deutschland, 29/11/2019
Effects of extracellular vesicles from blood derivatives on osteoarthritic chondrocytes
ASEV/GSEV Joint Meeting, Freising, Deutschland, 28/11/2019
Anti-coagulation influences properties of extracellular vesicles isolated from autologous blood derived products used for cartilage repair
World Congress of the International cartilage repair and joint preservation society, Vancouver, Kanada, 08/10/2019
Coagulation influences properties of extracellular vesicles isolated from autologous blood derived products
TERMIS EU Chapter, Rhodos, Griechenland, 28/05/2019
Efficient clearance of lipoproteins from anti-coagulated and native blood derived products to yield pure extracellular vesicle preparations
TERMIS-EU, Rhodos, Griechenland, 28/05/2019
Efficient clearance of lipoproteins from anti-coagulated and native blood-derived products to yield pure extracellular vesicle preparations
ISEV, Kyoto, Japan, 26/04/2019
Blood derived products contain extracellular vesicles with distinct biological properties for regenerative approaches
Word Congress of the International Society of Extracellular Vesicles (ISEV), Kyoto, Japan, 25/04/2019
Characterisation of extracellular vesicles in different blood derived products for osteoarthritis therapy
ESAO Winter School, Baden, Österreich, 24/01/2019
Different production technology of blood derived products influences differentiation pathway of various cell populations
World Congress of the International Society of Tissue Engineering and Regenerative Medicine (TERMIS), Kyoto, Japan, 07/09/2018
Different production technology of blood derived products influences differentiation pathway of various cell populations
Gordon Research Conference on Extracellular Vesicles, Newry, USA, 21/08/2018
Different production technology of blood derived products influences differentiation pathway of various cell populations
14. World congress of the International cartilage repair society (ICRS) , Macau, China, 12/04/2018
